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Total tracers:   36
By Application: Oncology 15   By Isotope: Tc-99m 14
  Neurology 12     Ga-67/In-111 4
  Cardiology 12     I-123/I-125 17
  Nephrology 3     Tl-201 1

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Id SPECT Tracer Database
11
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Chemical name:99mTc-sestamibi, 99mTc-methoxy-isobutyl-isonitrile
Common names:99mTc-MIBI
Commercial product:Cardiolite (BMS), Cardio-Spect (Medi-Radiopharma)
Supplier:Bristol Myers Squibb Medical Imaging, Medi-Radiopharma
Target:Cardiac metabolism
Mechanism:Metabolism-imaging radiopharmaceutical
Application:Oncology  Cardiology  
Reported Use: 
Add. Comments: 
Isotope Family:Tc-99m  
Publications:
Synthesis:
  • Cooper M, Dustan K, Rotureau L. The effect on radiochemical purity of modifications to the method of preparation and dilution of 99mTc-sestamibi. Nucl Med Commun. 2006 May;27(5):455-60.
  • Hung JC, Herold TJ, Wilson ME, Gibbons RJ. Generator eluate effects on the labeling efficiency of 99mTc-sestamibi. Nucl Med Biol. 1995 Oct;22(7):949-51.
  • Hung JC, Wilson ME, Brown ML, Gibbons RJ. Rapid preparation and quality control method for technetium-99m-2-methoxy isobutyl isonitrile (technetium-99m-sestamibi). J Nucl Med. 1991 Nov;32(11):2162-8.
  • Hung JC, Herold TJ, Gibbons RJ. Optimal conditions of 99mTc eluate for the radiolabeling of 99mTc-sestamibi. Nucl Med Biol. 1996 Jul;23(5):599-603.
  • In vitro analysis:
  • Mubashar M, Harrington KJ, Chaudhary KS, Lalani el-N, Stamp GW, Peters AM. Differential effects of toremifene on doxorubicin, vinblastine and Tc-99m-sestamibi in P-glycoprotein-expressing breast and head and neck cancer cell lines. Acta Oncol. 2004;43(5):443-52 .
  • Bernard BF, Krenning EP, Breeman WA, Ensing G, Benjamins H, Bakker WH, Visser TJ, de Jong M. 99mTc-MIBI, 99mTc-tetrofosmin and 99mTc-Q12 in vitro and in vivo.Nucl Med Biol. 1998 Apr;25(3):233-40.
  • Rodrigues M, Chehne F, Kalinowska W, Berghammer P, Zielinski C, Sinzinger H. Uptake of 99mTc-MIBI and 99mTc-tetrofosmin into malignant versus nonmalignant breast cell lines. J Nucl Med. 2000 Sep;41(9):1495-9.
  • Arbab AS, Koizumi K, Toyama K, Arai T, Araki T. Technetium-99m-tetrofosmin, technetium-99m-MIBI and thallium-201 uptake in rat myocardial cells. J Nucl Med. 1998 Feb;39(2):266-71.
  • Marian T, Szabo G, Goda K, Nagy H, Szincsak N, Juhasz I, Galuska L, Balkay L, Mikecz P, Tron L, Krasznai Z. In vivo and in vitro multitracer analyses of P-glycopr...
  • Animal Studies:
  • Maskali F, Franken PR, Poussier S, Tran N, Vanhove C, Boutley H, Le Gall H, Karcher G, Zannad F, Lacolley P, Marie PY.Initial infarct size predicts subsequent cardiac remodeling in the rat infarct model: an in vivo serial pinhole gated SPECT study. J Nucl Med. 2006 Feb;47(2):337-44.
  • Bernard BF, Krenning EP, Breeman WA, Ensing G, Benjamins H, Bakker WH, Visser TJ, de Jong M. 99mTc-MIBI, 99mTc-tetrofosmin and 99mTc-Q12 in vitro and in vivo.Nucl Med Biol. 1998 Apr;25(3):233-40.
  • Takahashi N, Reinhardt CP, Marcel R, Leppo JA. Myocardial uptake of 99mTc-tetrofosmin, sestamibi, and 201Tl in a model of acute coronary reperfusion. Circulation. 1996 Nov 15;94(10):2605-13.
  • Wang JH, Scollard DA, Teng S, Reilly RM, Piquette-Miller M. Detection of P-glycoprotein activity in endotoxemic rats by 99mTc-sestamibi imaging. J Nucl Med. 2005 Sep;46(9):1537-45.
  • Clinical Studies:
  • Lumachi F, Ferretti G, Povolato M, Bui F, Cecchin D, Marzola MC, Zucchetta P, Basso U. Axillary lymph node metastases detection with 99mTc-sestamibi scintimammography in patients with breast cancer undergoing curative surgery. Anticancer Res. 2007 Jul-Aug;27(4C):2949-52.
  • Adams GL, Shaw LK, Tuttle RH, Hanson MW, Pagnanelli R, Borges-Neto S. Prediction of mortality in patients with coronary artery disease undergoing vasodilator stress testing: a comparison between 99mTc-tetrofosmin and 99mTc-sestamibi. Nucl Med Commun. 2007 Jun;28(6):457-63.
  • Reyes E, Loong CY, Harbinson M, Rahman S, Prvulovich E, Ell PJ, Anagnostopoulos C, Underwood SR. A comparison of Tl-201, Tc-99m sestamibi, and Tc-99m tetrofosmin myocardial perfusion scintigraphy in patients with mild to moderate coronary stenosis. J Nucl Cardiol. 2006 Jul;13(4):488-94.
  • Kakhki VR, Sadeghi R, Zakavi SR. Assessment of transient left ventricular dilation ratio via 2-day dipyridamole Tc-99m sestamibi nongated myocardial perfusion imaging. J Nucl Ca...
  • Examples
    Dual-Isotope ECG-Gated SPECT/CT of a Rat
    2009-10-14
    University of New Mexico
    Cardiac-gated mouse heart
    2008-07-08
    Mediso, Ltd.
    Respiratory Gated Mouse (All views)
    2008-07-03
    University of New Mexico
    Mouse Cardiac/Thyroid Dual-Isotope Scan (Slices, Tc-99m/MIBI, I-123)
    2007-02-26
    University of New Mexico
    Cardiac Mouse Study (Tc-99m MIBI)
    2006-11-18
    Erasmus MC, Rotterdam
    Respiratory Gated Mouse (Coronal view with CT)
    2008-07-03
    University of New Mexico
    Mouse Cardiac/Thyroid Dual-Isotope Scan (Fusion, Tc-99m/MIBI, I-123)
    2007-02-26
    University of New Mexico
    Cardiac Mouse Study (Tc-99m MIBI, transverse)
    2006-11-18
    Erasmus MC, Rotterdam
    Cardiac Mouse Study (Tc-99m MIBI, transverse)
    2006-11-18
    Erasmus MC, Rotterdam
    Cardiac Mouse Study (Tc-99m MIBI, coronal)
    2006-11-18
    Erasmus MC, Rotterdam
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